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sureselect methyl-seq platform  (Agilent technologies)


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    Structured Review

    Agilent technologies sureselect methyl-seq platform
    Design and workflow for simultaneous interrogation of genetic variation and DNA methylation at HLA class II locus and immune gene promoters. (A) Chromosome representation of the targeted HLA class II locus. Genes are depicted in blue, while pseudogenes and non-coding RNAs are represented in green. (B) Illustration of DNA fragment capture using biotin-labelled RNA probes based on the Agilent <t>SureSelect</t> Methyl-Seq Capture method. (C) Workflow for the parallel preparation of variant and methylation libraries adapted from SureSelect Methyl-Seq. (D) Bioinformatic pipeline used for the analysis of the variant and methylation libraries.
    Sureselect Methyl Seq Platform, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sureselect methyl-seq platform/product/Agilent technologies
    Average 90 stars, based on 1 article reviews
    sureselect methyl-seq platform - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "Simultaneous detection of DNA variation and methylation at HLA class II locus and immune gene promoters using targeted SureSelect Methyl-Sequencing"

    Article Title: Simultaneous detection of DNA variation and methylation at HLA class II locus and immune gene promoters using targeted SureSelect Methyl-Sequencing

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2023.1251772

    Design and workflow for simultaneous interrogation of genetic variation and DNA methylation at HLA class II locus and immune gene promoters. (A) Chromosome representation of the targeted HLA class II locus. Genes are depicted in blue, while pseudogenes and non-coding RNAs are represented in green. (B) Illustration of DNA fragment capture using biotin-labelled RNA probes based on the Agilent SureSelect Methyl-Seq Capture method. (C) Workflow for the parallel preparation of variant and methylation libraries adapted from SureSelect Methyl-Seq. (D) Bioinformatic pipeline used for the analysis of the variant and methylation libraries.
    Figure Legend Snippet: Design and workflow for simultaneous interrogation of genetic variation and DNA methylation at HLA class II locus and immune gene promoters. (A) Chromosome representation of the targeted HLA class II locus. Genes are depicted in blue, while pseudogenes and non-coding RNAs are represented in green. (B) Illustration of DNA fragment capture using biotin-labelled RNA probes based on the Agilent SureSelect Methyl-Seq Capture method. (C) Workflow for the parallel preparation of variant and methylation libraries adapted from SureSelect Methyl-Seq. (D) Bioinformatic pipeline used for the analysis of the variant and methylation libraries.

    Techniques Used: DNA Methylation Assay, Variant Assay, Methylation



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    Design and workflow for simultaneous interrogation of genetic variation and DNA methylation at HLA class II locus and immune gene promoters. (A) Chromosome representation of the targeted HLA class II locus. Genes are depicted in blue, while pseudogenes and non-coding RNAs are represented in green. (B) Illustration of DNA fragment capture using biotin-labelled RNA probes based on the Agilent SureSelect Methyl-Seq Capture method. (C) Workflow for the parallel preparation of variant and methylation libraries adapted from SureSelect Methyl-Seq. (D) Bioinformatic pipeline used for the analysis of the variant and methylation libraries.

    Journal: Frontiers in Immunology

    Article Title: Simultaneous detection of DNA variation and methylation at HLA class II locus and immune gene promoters using targeted SureSelect Methyl-Sequencing

    doi: 10.3389/fimmu.2023.1251772

    Figure Lengend Snippet: Design and workflow for simultaneous interrogation of genetic variation and DNA methylation at HLA class II locus and immune gene promoters. (A) Chromosome representation of the targeted HLA class II locus. Genes are depicted in blue, while pseudogenes and non-coding RNAs are represented in green. (B) Illustration of DNA fragment capture using biotin-labelled RNA probes based on the Agilent SureSelect Methyl-Seq Capture method. (C) Workflow for the parallel preparation of variant and methylation libraries adapted from SureSelect Methyl-Seq. (D) Bioinformatic pipeline used for the analysis of the variant and methylation libraries.

    Article Snippet: To address these limitations and enable investigation of the class II locus, we developed a targeted-capture approach based on the Agilent SureSelect Methyl-Seq platform for simultaneous investigation of genetic and epigenetic variations of the HLA class II locus.

    Techniques: DNA Methylation Assay, Variant Assay, Methylation